List of top Biotechnology Questions on Recombinant DNA technology and applications

Describe the basic steps of Recombinant DNA Technology.

Explain the method of Blue-White selection used for screening of recombinant cells containing desired plasmid with gene of interest.

Outline the important steps for isolation of recombinant insulin (Humulin) from Escherichia coli.

The DNA ligase enzyme that is frequently used to ligate different DNA fragments in order to generate rDNA molecules is isolated from:

To perform DNA sequencing of a strand, we need to clone the sequence in a single-stranded form. Which vector will you prefer for this ?

Give the names of any three genes that are used as selectable markers in recombinant DNA technology. Also mention the trait/protein they specify.

Illustrate steps to show the construction of a recombinant DNA molecule.

Identify the vector that infects E. coli cells containing F-plasmid and that has a single-stranded circular genome:}