Match the following.
\[ \begin{array}{|c|l|c|l|} \hline & \textbf{Disease / Application} & & \textbf{Molecular Basis / Use} \\ \hline A & \text{Sickle Cell Anemia} & I & \text{Frameshift Mutation} \\ B & \text{Thalassemia} & II & \text{Missense Point Mutation} \\ C & \text{Duchenne Muscular Dystrophy} & III & \beta\text{-globin Gene Defect} \\ D & \text{PCR} & IV & \text{DNA Amplification Technique} \\ \hline \end{array} \]
The correct matching is