List of top Botany Questions on Biotechnology asked in AP EAPCET

In pBR 322 plasmid the tetracycline antibiotic resistance gene has the recognition site for which of the following restriction enzymes.
I. PVU I
II. Sal I
III. BamH I
IV. Pst I

During the isolation of desired gene from the fungal cell which enzyme is not used?

Choose the incorrect statements: A) Alien DNA can be linked to origin of replication of DNA. B) DNA ligase can act as a restriction enzyme. C) Each restriction endonuclease recognizes a specific palindromic nucleotide sequence in DNA. D) Exonucleases will cut the specific position of DNA.

Choose the correct statements among the following: A) Selection of recombinant DNA by the inactivation of antibiotics is a cumbersome process. B) Formation of chimeric DNA is possible when cutting the DNA by a restriction enzyme and by adding ligase. C) Any protein-encoding gene expressed in a heterologous host is called a recombinant protein. D) The probability that GAATTC occurs in DNA is once in 4196 nucleotides.

Restriction enzyme digestion means

‘Ti’ plasmid of which bacterium is used for obtaining Transgenic plants

A plant having altered gene from a different species called

Two enzymes responsible for restricting the growth of bacteriophage were isolated from

Statement 1: Plasmid DNA is circular
Statement 2: Plasmid possess single stranded DNA without polarity