Question:hard

Choose the incorrect statements: A) Alien DNA can be linked to origin of replication of DNA. B) DNA ligase can act as a restriction enzyme. C) Each restriction endonuclease recognizes a specific palindromic nucleotide sequence in DNA. D) Exonucleases will cut the specific position of DNA.

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Ligase = Glue; Restriction Enzyme = Scissors (Endonuclease); Exonuclease = Trimmer (cuts from ends).
Updated On: Jun 10, 2026
  • A and B
  • B and C
  • A and C
  • B and D
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The Correct Option is D

Solution and Explanation

Step 1: Understand the question.
We must pick the two incorrect statements about recombinant DNA tools. So we look for the statements that are false.

Step 2: Check statement A.
A says alien DNA can be joined to the origin of replication. This is correct, because a piece of foreign DNA must be linked to an ori so it can copy itself inside the host. A is true, so it is not an answer.

Step 3: Check statement B.
B says DNA ligase can act as a restriction enzyme. This is wrong. Ligase joins DNA pieces together, while a restriction enzyme cuts DNA. They do opposite jobs. So B is incorrect.

Step 4: Check statement C.
C says each restriction endonuclease recognises a specific palindromic sequence. This is correct. These enzymes read a particular palindrome and cut there. C is true, so it is not an answer.

Step 5: Check statement D.
D says exonucleases cut at a specific position of DNA. This is wrong. Exonucleases trim nucleotides from the ends, while it is the endonucleases that cut at specific internal sites. So D is incorrect.

Step 6: Pick the incorrect set.
The two wrong statements are B and D.
\[ \boxed{\text{B and D}} \]
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