Question

During the isolation of desired gene from the fungal cell which enzyme is not used?

• A. Chitinase
• B. RNase
• C. Lysozyme
• D. Protease

Hint:

Cell wall digestion enzymes:
Bacteria = Lysozyme.
Fungi = Chitinase.
Plants = Cellulase and Pectinase.
Matching the enzyme to the cell wall chemistry helps solve these questions easily.

Answer 1

The Correct Option is C

Step 1: List what needs breaking down to get pure DNA.
To free genomic DNA from a fungal cell, you must break the cell wall and then clear away contaminating protein and RNA.
Step 2: Match each enzyme to its target.
Chitinase breaks down the chitin that makes up the fungal cell wall. RNase clears out RNA. Protease digests the contaminating proteins. All three are needed.
Step 3: Spot the odd one out.
Lysozyme attacks peptidoglycan, the cell wall material found in bacteria, not the chitin wall of fungi, so it plays no role here.
Final answer: Option 3, Lysozyme.