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Why the absorption of UV light by double stranded DNA increases (hyperchromic effect) when the DNA is denatured?

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Stacked bases hide from light $\rightarrow$ lower absorption. Unstacked bases (denatured single strands) are fully exposed $\rightarrow$ higher absorption (Hyperchromic shift).

Updated On: May 22, 2026

Due to loss of base stacking \
Due to increase in base stacking \
Due to renaturation \
Due to inhibition of light

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The Correct Option is A

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Why the absorption of UV light by double stranded DNA increases (hyperchromic effect) when the DNA is denatured?

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The Correct Option is A

Solution and Explanation

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