Question

Which technique is specifically used to detect the presence of a specific DNA sequence in a sample?

• A. Western blotting
• B. Southern blotting
• C. Northern blotting
• D. ELISA

Hint:

{Blotting rule:}
Southern → DNA
Northern → RNA
Western → Protein

Answer 1

The Correct Option is B

Topic: Molecular Biology Techniques
Blotting techniques allow researchers to identify specific molecules within a complex mixture by transferring them to a membrane and using a probe.
Steps:
Understanding the Question: We need to match the specific "Direction" of the blot name (Southern, Northern, Western) to the molecule it detects (DNA, RNA, Protein).
Key Formulas and Approach: Use the SNOW DROP mnemonic.
Detailed Solution:
Southern Blotting (named after Edwin Southern) is used for DNA.
Northern Blotting is used for RNA.
Western Blotting is used for Proteins.
ELISA is an antibody-based detection method, usually for proteins/antigens, but not a "blotting" technique.