Question

Which one of the following statements is correct in the context of observing DNA separation by agarose gel electrophoresis?

• A. DNA can be seen in visible light.
• B. DNA can be seen without staining in visible light.
• C. Ethidium bromide stained DNA can be seen in visible light.
• D. Ethidium bromide stained DNA can be seen under UV light.

Hint:

Ethidium bromide is a commonly used dye in molecular biology for visualizing DNA under UV light during electrophoresis.

Answer 1

The Correct Option is D

Ethidium bromide, a fluorescent dye that intercalates into DNA, enables visualization of DNA bands post-agarose gel electrophoresis when excited by UV light, causing it to fluoresce.