Question

Which of the following is not an application of $PCR$ (Polymerase Chain Reaction)?

• A. Molecular diagnosis
• B. Gene amplification
• C. Purification of isolated protein
• D. Detection of gene mutation

Answer 1

The Correct Option is C

The Polymerase Chain Reaction (PCR) is a powerful and widely-used technique in molecular biology. It is used for the amplification of DNA sequences and has several important applications:

1. Molecular diagnosis: PCR is used to diagnose diseases by amplifying the DNA of pathogens, making it easier to identify and study them. This method is particularly useful in detecting viral and bacterial infections.
2. Gene amplification: The primary function of PCR is to amplify specific DNA sequences from a small sample, producing millions of copies. This is crucial for further analysis and study in a laboratory setting.
3. Detection of gene mutation: PCR can be used to detect mutations in genes, which may lead to certain diseases. It allows for the comparison of mutated genes against normal ones, helping in genetic studies and medical diagnostics.

On the other hand, Purification of isolated protein is not an application of PCR. Protein purification requires different techniques and processes, such as chromatography, electrophoresis, and centrifugation, which separate proteins based on their unique properties, such as size, charge, and solubility.

Therefore, the correct answer is:

Purification of isolated protein