Question

Which metal ion is required during PCR?

• A. Magnesium (Mg\(^{2+}\))
• B. Calcium (Ca\(^{2+}\))
• C. Potassium (K\(^{+}\))
• D. Sodium (Na\(^{+}\))

Hint:

In PCR, magnesium ions are critical for enzyme activation and efficient DNA synthesis. Too much or too little can hinder the reaction.

Answer 1

The Correct Option is A

Polymerase Chain Reaction (PCR) amplifies DNA. DNA polymerase, the enzyme that synthesizes new DNA strands, requires magnesium ions (Mg\(^{2+}\)) for activity. These ions stabilize DNA polymerase and facilitate nucleotide binding to the elongating DNA strand during PCR. Consequently, magnesium (Mg\(^{2+}\)) is the essential metal ion for PCR.