Question:medium

Which of the following enzymes under appropriate conditions produce single-stranded nicks in DNA?

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DNase I + Magnesium ($\text{Mg}^{2+}$) = Independent single-strand nicks. DNase I + Manganese ($\text{Mn}^{2+}$) = Full double-strand breaks. The metal cofactor completely changes how it cuts!

Updated On: May 22, 2026

S1 nuclease \
Exonuclease III \
DNase I \
Terminal transferase

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The Correct Option is C

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Which of the following enzymes under appropriate conditions produce single-stranded nicks in DNA?

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The Correct Option is C

Solution and Explanation

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