Question

Which enzyme is used in Sanger’s method of DNA sequencing?

Hint:

DNA polymerase extends the primer by adding nucleotides and is essential for DNA synthesis in Sanger sequencing.

Answer 1

Sanger sequencing employs DNA polymerase to synthesize a new DNA strand. This enzyme adds nucleotides complementary to the template, incorporating both standard deoxynucleotides (dNTPs) and chain-terminating dideoxynucleotides (ddNTPs). This differential incorporation facilitates sequence determination through chain termination.