Question:medium

Which algorithm is used for performing Global Sequence Alignment?

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Global Sequence Alignment → {Needleman–Wunsch Algorithm}
Local Sequence Alignment → {Smith–Waterman Algorithm}
Updated On: Mar 17, 2026
  • Smith–Waterman Algorithm
  • Needleman–Wunsch Algorithm
  • BLAST Algorithm
  • FASTA Algorithm
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The Correct Option is B

Solution and Explanation

This question addresses a core concept in bioinformatics: sequence alignment. The goal of sequence alignment is to arrange DNA, RNA, or protein sequences to identify regions of similarity that may be a consequence of functional, structural, or evolutionary relationships. Alignments can be performed globally, comparing sequences across their entire length, or locally, to find the best-matching segments within them.
Understanding the Question The question asks to identify the specific algorithm designed for performing a global alignment of two sequences.
Key Concepts and Approach The key is to distinguish between global and local alignment algorithms. The approach involves matching the type of alignment with its corresponding foundational algorithm.
Detailed Solution
Global Alignment Goal: Global sequence alignment attempts to find the best possible alignment between two sequences from end to end. This method is most suitable when comparing two sequences that are similar in length and are expected to share similarity across their entire span.
The Needleman–Wunsch Algorithm: This is the classic dynamic programming algorithm developed specifically for global alignment. It constructs a matrix to score all possible alignments and then traces back from the final cell to find the optimal alignment path that maximizes the score.
Contrasting Other Options:
The Smith–Waterman algorithm is used for local alignment, finding the most similar subsequences.
BLAST and FASTA are heuristic algorithms designed for rapid similarity searches in large databases, not for computing the mathematically optimal global alignment.

Conclusion: Therefore, the Needleman–Wunsch algorithm is the standard method for global sequence alignment.
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