Transcription is the synthesis of mRNA from a DNA template. This process occurs in the 5' to 3' direction, yielding an RNA sequence complementary to the DNA template. Uracil (U) substitutes thymine (T) in RNA; therefore, the mRNA sequence is complementary to the DNA template sequence, with T replaced by U. For instance, given a DNA template strand of 3'-TACGCT-5', the resultant mRNA sequence is 5'-AUGCGA-3', due to uracil (U) replacing thymine (T) in RNA. Consequently, the correct answer is 5'-AUGCGA-3'.