Question

Transfer of DNA strands from agarose gel to nylon membrane is known as:

• A. Southern blotting
• B. Northern blotting
• C. Western blotting
• D. Eastern blotting

Hint:

Remember the biological matching for blotting:
Southern = DNA
Northern = RNA
Western = Protein

Answer 1

The Correct Option is A

Step 1: Recall the family of blotting techniques.
Molecular biology uses different blotting names depending on which molecule is being transferred and detected, DNA, RNA, or protein.
Step 2: Match the molecule in the question.
The question specifically talks about DNA fragments moving from an agarose gel onto a nylon membrane.
Step 3: Name the technique.
This DNA specific transfer method was developed by Edwin Southern and is called Southern blotting, RNA transfer would instead be called Northern blotting and protein transfer Western blotting.
Final answer: Option 1, Southern blotting.