Question

The question consist of two statements – Assertion (A) and Reason (R). Answer these questions selecting the appropriate option given below:

• A. Both (A) and (R) are true and (R) is the correct explanation of (A).
• B. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
• C. (A) is true, but (R) is false.
• D. (A) is false, but (R) is true.
  Assertion (A): In molecular diagnosis, single-stranded DNA or RNA tagged with a radioactive molecule is called a probe.
  Reason (R): A probe always searches and hybridises with its complementary DNA in a clone of cells.

Hint:

Probes are crucial in techniques like Southern blotting and in situ hybridisation for detecting specific DNA or RNA sequences.

Answer 1

The Correct Option is A

Probes are single-stranded DNA or RNA molecules, labeled radioactively or fluorescently, which hybridize to complementary sequences in target DNA during molecular diagnostics. This process ensures high specificity for the detection of genetic abnormalities.