If the sequence of nitrogen bases of the coding strand in a transcription unit is 5\(\prime\)–ATGAATG–3\(\prime\), the sequence of bases in its RNA transcript would be:
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During transcription, RNA is synthesized using the DNA template strand, but its sequence matches the coding strand with uracil substituted for thymine.
The RNA transcript is complementary to the DNA template strand and matches the DNA coding strand, with uracil substituted for thymine. \[\text{Coding strand: } 5\(\prime\)–ATGAATG–3\(\prime\) \text{RNA transcript: } 5\(\prime\)–AUGAAUG–3\(\prime\)\]