Question

The function of a primer is in?

• A. Transcription
• B. Translation
• C. Initiation of DNA replication
• D. Termination of DNA replication

Hint:

DNA polymerase needs a free 3-prime end to start; what supplies it?

Answer 1

The Correct Option is C

Step 1: Consider the limitation of DNA polymerase: it requires a free 3-prime OH to extend and cannot make the very first phosphodiester bond between two free nucleotides.

Step 2: Nature solves this by having primase synthesize a short RNA stretch complementary to the template; this RNA gives the 3-prime OH that DNA polymerase needs to begin.

Step 3: Because the primer supplies the starting point for new strand synthesis, its role is to kick off (initiate) DNA replication. It is unrelated to transcription, translation, or chain termination.

\[\boxed{\text{Initiation of DNA replication}}\]