This question tests whether you remember the exact reagent, pH and soil to solution ratio fixed for each standard soil test, since a small change in any one of these numbers turns a correct method into a wrong one.
- (A) 2 M KCl for mineral N at 1:10: Correct. Potassium chloride solution pulls out both ammonium and nitrate forms of nitrogen, and the fixed ratio for this test is 1:10.
- (B) DTPA at pH 7.5 for micronutrients at 1:20: Wrong. The DTPA test for zinc, copper, iron and manganese runs at pH 7.3, and the soil to solution ratio is 1:2, not 1:20, so both numbers here are off.
- (C) Ammonium acetate (1 N) for potassium at 1:5: Correct. Neutral normal ammonium acetate at a 1:5 ratio is the accepted method for available potassium.
- (D) Olsen reagent for available P at 1:20: Correct. Olsen's 0.5 M sodium bicarbonate at pH 8.5 is used for available phosphorus, at the standard 1:20 ratio.
Since (B) carries a wrong pH and a wrong ratio while (A), (C) and (D) all state the correct reagent, pH and ratio, only (A), (C) and (D) hold true together.
Let's summarize:
- DTPA works at pH 7.3 with a 1:2 soil to solution ratio, not pH 7.5 or 1:20.
- KCl for nitrogen, ammonium acetate for potassium and Olsen reagent for phosphorus are all stated correctly in the question.
So the answer is (A), (C) and (D) only, option 4.