To determine the correct statements with reference to chordates, let's analyze each statement provided:
- Presence of a mid-dorsal, solid and double nerve cord: This statement is incorrect. Chordates are characterized by a dorsal, hollow, single nerve cord.
- Presence of closed circulatory system: This statement is correct. Chordates typically have a closed circulatory system where blood flows within enclosed vessels.
- Presence of paired pharyngeal gill slits: This statement is correct. Most chordates have pharyngeal gill slits at some stage during their development.
- Presence of dorsal heart: This statement is incorrect. Chordates generally have a ventral heart, not a dorsal one.
- Triploblastic pseudocoelomate animals: This statement is incorrect. Chordates are triploblastic, but they are coelomates, not pseudocoelomates.
Therefore, the correct statements with reference to chordates are:
B. Presence of closed circulatory system and C. Presence of paired pharyngeal gill slits.
Thus, the correct answer is "B and C only".