Question

Mention the correct sequence of events for the isolation of protoplasts from embryogenic suspension cultures of rice.
A. Excise yellow/white compact and nodular embryogenic callus
B. Staining with fluorescein diacetate
C. Filter the cell suspension through a nylon sieve
D. Filter the protoplast suspension through sieves
Choose the correct answer from the options given below:

• A. A, B, D, C
• B. A, C, D, B
• C. C, B, A, D
• D. C, D, B, A

Hint:

The logical flow is: Pick the tissue (A) $\rightarrow$ Clean the clumps (C) $\rightarrow$ Filter the product (D) $\rightarrow$ Check if they are alive (B).

Answer 1

The Correct Option is B