Question

Match LIST-I with LIST-II
\begin{tabularx}{0.9\textwidth}{|l|X|l|X|} \hline \multicolumn{2}{|c|}{LIST-I} & \multicolumn{2}{c|}{LIST-II}
\multicolumn{2}{|c|}{Replication Enzymes in E coli} & \multicolumn{2}{c|}{Replication Enzymes in Human}
\hline A. & \(\beta\)-clamp & I. & RPA
B. & SSB & II. & PCNA
C. & Gyrase & III. & DNA polymerase alpha
D. & DnaG & IV. & Topo-I, II
\hline \end{tabularx}
Choose the most appropriate answer from the options given below:

• A. A - I, B - II, C - III, D - IV
• B. A - I, B - III, C - II, D - IV
• C. A - I, B - II, C - IV, D - III
• D. A - II, B - I, C - IV, D - III

Hint:

To remember the sliding clamps: think of the Greek letter Beta (\(\beta\)) for \textbf{B}acteria ({E. coli}) and PCNA, which sounds like a clinical term, for more complex organisms like humans.

Answer 1

The Correct Option is D

Step 1: Concept Overview:
This question involves matching key prokaryotic ({E. coli}) DNA replication proteins with their eukaryotic (Human) functional counterparts. While protein names differ, their roles are conserved.

Step 2: Detailed Matching:
- A. \(\beta\)-clamp: The {E. coli} sliding clamp ensures DNA polymerase processivity. Its human homolog is PCNA (Proliferating Cell Nuclear Antigen). Therefore, A corresponds to II.
- B. SSB (Single-Strand Binding protein): In {E. coli}, SSB prevents single-stranded DNA reannealing and degradation. The human homolog is RPA (Replication Protein A). Thus, B aligns with I.
- C. Gyrase: This {E. coli} type II topoisomerase relieves torsional stress from helicase activity. In humans, Topoisomerases I and II (Topo-I, II) perform this function. Consequently, C pairs with IV.
- D. DnaG: The {E. coli} primase initiates DNA synthesis with short RNA primers. Human primase exists in a complex with DNA polymerase alpha, which synthesizes and extends the primer. Hence, D is matched with III.

Step 3: Solution:
The correct matches are A-II, B-I, C-IV, D-III, corresponding to option (D).