Question

Match LIST-I with LIST-II 

LIST-I		LIST-II
A.	Sea weeds	I.	Isolation of DNA from gel
B.	Staining of DNA	II.	Gel electrophoresis
C.	Elution	III.	Source of agarose
D.	Separation of DNA fragments	IV.	Ethidium bromide

Choose the correct answer from the options given below: 
 

• A. A-III, B-IV, C-II, D-I
• B. A-III, B-IV, C-I, D-II
• C. A-II, B-IV, C-I, D-III
• D. A-III, B-II, C-IV, D-I

Hint:

Visualize the entire gel electrophoresis process to answer these questions: You make the gel with agarose from {sea weeds} (A-III). You run the gel for {separation} (D-II). You {stain} with EtBr to see the DNA (B-IV). You cut out the band and {elute} it to get the pure DNA (C-I).

Answer 1

The Correct Option is B

Step 1: Concept Overview:
This question involves matching components and steps of agarose gel electrophoresis with their corresponding descriptions.
Step 2: Detailed Explanation:
A. Source of Agarose: Agarose, the gel matrix in electrophoresis, is derived from specific red III. sea weeds, such as those from the genera Gelidium and Gracilaria.
B. DNA Visualization: IV. Ethidium bromide, a fluorescent dye, is commonly used to stain DNA after electrophoresis. It intercalates into DNA and fluoresces under UV light, enabling visualization.
C. DNA Extraction: I. Isolation of DNA from gel, also known as elution, refers to the process of extracting and purifying a specific DNA fragment from the agarose gel after separation and visualization.
D. Fragment Separation: II. Gel electrophoresis is primarily used to separate macromolecules like DNA, RNA, and proteins. For DNA, separation is based on fragment size.
Step 3: Solution:
The correct matches are A-III, B-IV, C-I, D-II.