Question

Match LIST-I with LIST-II 

LIST-I (Technique)		LIST-II (Used for)
A.	Northern blotting	I.	To detect specific proteins in this sample of tissue homogenate
B.	Southern blotting	II.	Detection of specific post translation modification of proteins
C.	Western blotting	III.	To detect specific RNA molecule in mixture of RNA
D.	Eastern blotting	IV.	To detect specific DNA in a mix of samples

Choose the correct answer from the options given below: 
 

• A. A-II, B-III, C-IV, D-I
• B. A-III, B-IV, C-I, D-II
• C. A-III, B-IV, C-II, D-I
• D. A-I, B-II, C-III, D-IV

Hint:

Use the mnemonic {SNOW DROP} to remember the main blotting techniques: {S}outhern \(\rightarrow\) {D}NA {N}orthern \(\rightarrow\) {R}NA {O} \(\rightarrow\) {O} (Nothing) {W}estern \(\rightarrow\) {P}rotein

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
This question assesses understanding of various blotting techniques in molecular biology used to identify specific macromolecules.
Step 2: Detailed Explanation:
B. Southern blotting: Named after Edwin Southern, this method is used IV. To detect specific DNA sequences within a sample.
A. Northern blotting: Similar to Southern blotting, this technique is used III. To detect specific RNA molecules, often for gene expression studies.
C. Western blotting: This technique employs antibodies I. To detect specific proteins in a sample.
D. Eastern blotting: A less frequently used technique for the II. Detection of specific post-translational modifications of proteins, like glycosylation or phosphorylation.
Step 3: Final Answer:
The correct matches are A-III, B-IV, C-I, and D-II.