Question

Match LIST-I with LIST-II 

LIST-I		LIST-II
A.	Gene inhibition	I.	Addition of functional gene to their genome to replace missing product
B.	Gene editing	II.	Disarm the product of faulty gene
C.	Gene targeting	III.	CRISPR/Cas9
D.	Gene Augmentation therapy	IV.	Replacement of non-functional gene with normal gene

Choose the correct answer from the options given below: 
 

• A. A-III, B-IV, C-II, D-I
• B. A-II, B-III, C-IV, D-I
• C. A-I, B-II, C-III, D-IV
• D. A-IV, B-III, C-I, D-II

Hint:

Remember the core ideas: {Augmentation} = Adding a good copy. {Inhibition} = Stopping a bad copy. {Editing} = Making precise changes (think CRISPR). {Targeting/Replacement} = Swapping out a gene.

Answer 1

The Correct Option is B

Step 1: Concept Overview:
This question involves matching gene therapy approaches (LIST-I) with their corresponding descriptions or mechanisms (LIST-II).
Step 2: Detailed Explanation:
A. Gene inhibition: This strategy addresses diseases resulting from a faulty gene producing a harmful product (gain-of-function mutation). The objective is to halt or 'inhibit' the gene's activity, achieved by blocking transcription or translation. This aligns with II. Disarm the product of faulty gene.
B. Gene editing: This refers to technology enabling precise alterations to an organism's DNA sequence. The prominent tool for this is III. CRISPR/Cas9.
C. Gene targeting: This technique employs homologous recombination to modify an endogenous gene, enabling the creation of specific mutations, correction of faulty genes, or gene replacement. This corresponds to IV. Replacement of non functional gene with normal gene.
D. Gene Augmentation therapy: This common gene therapy approach addresses diseases caused by a gene function loss (e.g., cystic fibrosis). It introduces a healthy, functional gene copy into cells to compensate for the faulty one, matching I. Addition of functional gene to their genome to replace missing product.
Step 3: Solution:
The correct matches are: A-II, B-III, C-IV, D-I.