Question

Joining two DNA molecules using an adapter involves the following steps
A. Artificial synthesis of an adapter
B. Treating with polynucleotide kinase
C. Blunt end ligation to add an adapter to a blunt-ended DNA molecule
D. Joining two DNA molecules with sticky ends
What is the correct sequence in which these steps are performed?

• A. B, A, D, C
• B. A, C, B, D
• C. D, B, C, A
• D. C, A, D, B

Hint:

Adapters have "pre-existing" sticky ends. Linkers, by contrast, are blunt-ended and must be cut with a restriction enzyme after ligation to create sticky ends.

Answer 1

The Correct Option is B