Question:medium

In Polymerase Chain Reaction (PCR) primers are used to provide:

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Remember: D elongation always proceeds from $5'$ to $3'$.
A free $3'$-OH group is indispensable for phosphodiester bond formation during D replication and PCR amplifications.
Updated On: Jul 28, 2026
  • 3'-OH
  • 5'-OH
  • 3'-PO$_4^-$
  • 5'-PO$_4^-$
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The Correct Option is A

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