To address the question of identifying the correct statement among the given options, we need to examine each option in detail:
- **Split gene arrangement is characteristic of prokaryotes.**
- This statement is incorrect. Split genes, or genes interrupted by introns, are characteristic of eukaryotes, not prokaryotes. Prokaryotic genes are generally continuous sequences without introns.
- **In capping, methyl guanosine triphosphate is added to the 3' end of hnRNA.**
- This statement is incorrect. In the process of capping, a 7-methylguanylate cap is added to the 5' end of hnRNA (heterogeneous nuclear RNA) in eukaryotes, not the 3' end.
- **RNA polymerase binds with Rho factor to terminate the process of transcription in bacteria.**
- This statement is correct. In bacteria, Rho-dependent termination is one of the mechanisms used to terminate transcription. The Rho factor is a protein that binds to the RNA polymerase during transcription, facilitating the termination of transcription at specific sites.
- **The coding strand in a transcription unit is copied to an mRNA.**
- This statement is incorrect. During transcription, the template strand (not the coding strand) is used by RNA polymerase to synthesize mRNA. The mRNA sequence is complementary to the template strand and identical to the coding strand, except that thymine (T) is replaced with uracil (U).
Based on the explanations above, the correct answer is that RNA polymerase binds with Rho factor to terminate the process of transcription in bacteria.