Question

Given below are the sequence of events in the production of human insulin by Eli Lilly.
a) Preparation of two DNA sequences corresponding to chain A and B of human insulin.
b) Production of chain A and B separately.
c) Introduction of chains in plasmids of E.coli.
d) Extraction of chain A and B combining them by creating disulphide bonds.
Choose the correct sequence of events.

• A. (a), (b), (c), (d)
• B. (a), (c), (b), (d)
• C. (a), (b), (d), (c)
• D. (a), (d), (b), (c)

Hint:

You must put the genes into the bacteria (c) before the bacteria can produce the protein chains (b). This naturally orders a $\rightarrow$ c $\rightarrow$ b $\rightarrow$ d.

Answer 1

The Correct Option is B