Question

During tissue processing, what is the correct sequence of steps?

• A. Clearing, Dehydration, Infiltration
• B. Dehydration, Infiltration, Clearing
• C. Infiltration, Dehydration, Clearing
• D. Dehydration, Clearing, Infiltration

Hint:

Tissue processing order = Remove water (Dehydration) → Replace with clearing agent (Clearing) → Impregnate with wax (Infiltration).

Answer 1

The Correct Option is D

Phase 1: Review tissue preparation.
Tissue preparation aims to preserve structural integrity for microscopic analysis and facilitate embedding medium penetration.
Phase 2: Sequential workflow.
1. Dehydration: Water elimination via escalating alcohol concentrations.
2. Clearing: Alcohol substitution with a transparent agent (e.g., xylene).
3. Infiltration: Embedding medium (e.g., paraffin) permeation of the tissue.
Phase 3: Eliminate erroneous sequences.
- (A) Clearing cannot precede dehydration.
- (B) Infiltration must follow clearing.
- (C) Infiltration cannot precede dehydration.
- (D) Correct sequence: Dehydration → Clearing → Infiltration.
Phase 4: Final determination.
The correct sequence is Dehydration, Clearing, Infiltration.