Question

Arrange the given steps in BLAST procedure using a hypothetical Query in order of their occurrence. A. Calculate sum of match scores based on substitution matrix for the matched word, B. Find the database sequence corresponding to the best word match and extend alignment in both directions, C. Creation of every three residue words from the query sequence and scan them searching in BLAST word database, D. Determine high scored segment above threshold, E. Input query sequence.

• A. A, B, C, D, E
• B. A, E, B, C, D
• C. E, C, A, B, D
• D. E, D, C, B, A

Hint:

BLAST procedure starts with query input, then word generation, scoring, extension of alignment, and finally identification of high scoring segments.

Answer 1

The Correct Option is C