Question

Arrange the following in the correct sequence followed in Ultra Performance Liquid Chromatography.
A. Mobile phase reservoir
B. Guard column
C. Separation column
D. Detector

• A. A, B, C, D
• B. A, B, D, C
• C. B, A, D, C
• D. C, B, D, A

Hint:

Think of it like a train journey. The mobile phase is the train. It starts at the station (\textbf{Reservoir}), goes through a security check (\textbf{Guard Column}), travels the main route where passengers get off at different stops (\textbf{Separation Column}), and finally, the arrival of each passenger group is noted at the destination (\textbf{Detector}).

Answer 1

The Correct Option is A

The components of a chromatography system are ordered according to the fluid's flow.
Step 1: Initiation. The process begins with the solvent that carries the sample.
A. Mobile phase reservoir: This container holds the solvent (mobile phase) which is pumped through the system.

Step 2: Protection. The main separation column is protected before use.
B. Guard column: A small, disposable column is positioned before the main column to remove impurities and particles that could harm the separation column.

Step 3: Separation. This is where the sample components undergo chemical separation.
C. Separation column (or analytical column): This crucial component separates the mixture's components based on their interaction with the column's stationary phase.

Step 4: Detection. The separated components must be detected.
D. Detector: This instrument measures the components as they leave the column, generating a signal that forms a chromatogram.
The correct flow path sequence is A \(\rightarrow\) B \(\rightarrow\) C \(\rightarrow\) D.