Question

Antisense RNA technique is used -

• A. To silence the gene expression
• B. To enhance the gene expression
• C. For cell mediated gene transfer
• D. For DNA fingerprinting

Hint:

Think of "antisense" as being "anti-message." It binds to the messenger RNA and stops the message from being read, thus silencing the gene.

Answer 1

The Correct Option is A

Step 1: Concept Overview:
The question concerns the main use of antisense RNA. This method utilizes an RNA molecule that is complementary to a specific mRNA.
Step 2: In-depth Explanation:
The central dogma posits DNA is transcribed to mRNA, then translated to protein.
Antisense RNA is designed with a sequence that's the reverse complement of the mRNA.
When antisense RNA enters a cell, it binds to its target mRNA, creating a double-stranded RNA structure.
This structure is untranslatable by ribosomes and is often quickly broken down by enzymes like RNase H.
By blocking mRNA translation, antisense RNA effectively stops or silences the target gene's expression. The Flavr Savr tomato, where the technique slowed ripening, is a key example.
Step 3: Conclusion:
Antisense RNA binds to and inhibits mRNA translation, thus silencing gene expression.