Question

A Thermal Cycler/Stabilizer is used for .........?

• A. Maintaining temperature
• B. DNA amplification (PCR)
• C. Protein synthesis
• D. RNA transcription

Hint:

Thermal cyclers are essential for PCR, which is a fundamental technique in genetics, forensics, and diagnostics.

Answer 1

The Correct Option is B

A thermal cycler (PCR machine) is a molecular biology instrument employed for DNA amplification via Polymerase Chain Reaction (PCR).
The process entails: 
Denaturation: DNA heating to \(94-96^\circ \text{C}\) to separate strands. 
Annealing: Cooling to \(50-65^\circ \text{C}\) for primer binding to specific sequences. 
Extension: Temperature increase to \(72^\circ \text{C}\) enabling DNA polymerase to synthesize new DNA strands. Repetition of these cycles leads to exponential DNA amplification. 

Final Answer: \[ \boxed{\text{DNA amplification (PCR)}} \]