Question

5\(\prime\)–G\(^\downarrow\)AATTC–3\(\prime\)
3\(\prime\)–CTTAA\(^\uparrow\)G–5\(\prime\)
(a) Name the restriction enzyme that recognises the given specific sequence of bases. What are such sequences of bases referred to as?
(b) What are the arrows in the given figure indicating? Write the result obtained thereafter.

Hint:

Palindromic sequences are unique DNA sequences that read the same in both directions, enabling precise enzymatic cuts.

Answer 1

(a) Restriction enzyme: EcoRI. The sequences are palindromic. (b) The arrows denote the EcoRI restriction enzyme's cleavage site on the DNA. Result: The DNA is cleaved into fragments possessing sticky ends, facilitating foreign DNA insertion in genetic engineering.