Question

A technique for transferring denatured DNA molecules that have been separated electrophoretically from a gel to a matrix (such as a nitrocellulose or nylon membrane) on which a hybridization assay can be performed is called

• A. Northern blotting
• B. Southern blotting
• C. Eastern blotting
• D. Western blotting

Hint:

Use the mnemonic \textbf{S-N-O-W D-R-O-P}:

\textbf{S}outhern $\rightarrow$ \textbf{D}NA
\textbf{N}orthern $\rightarrow$ \textbf{R}NA
\textbf{O} $\rightarrow$ \textbf{O} (nothing)
\textbf{W}estern $\rightarrow$ \textbf{P}rotein
This helps you remember which blotting technique corresponds to which macromolecule.

Answer 1

The Correct Option is B

Step 1: Concept Overview:
Blotting methods involve transferring macromolecules (DNA, RNA, or proteins) from an electrophoresis gel onto a solid membrane for detection.
Step 2: Technique Breakdown:
Blotting techniques are named based on the molecule they target:


Southern blotting: Detects specific DNA sequences. Separating DNA via gel electrophoresis and transferring it to a membrane for hybridization defines Southern blotting.

Northern blotting: Detects specific RNA sequences.

Western blotting: Detects specific proteins using antibodies.

Eastern blotting: Detects post-translational modifications (less common).

Step 3: Conclusion:
Southern blotting is the method used to transfer separated DNA from a gel to a membrane.