Question:medium

A researcher wants to clone a large DNA fragment \((\sim 40 kb)\) and express it in E. coli. The fragment must replicate autonomously, allow selection of recombinants and come from bacteriophage origin. Which vector is most appropriate?

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For cloning large DNA fragments around \(40 kb\), cosmids are suitable because they combine plasmid features with phage cos sites.
Updated On: May 18, 2026
  • pUC Plasmid
  • M13 Phage vector
  • Cosmid shuttle vector
  • Phage vector
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The Correct Option is C

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