Step 1 (a):
Alien species invasion: Introduction of Nile perch in Lake Victoria caused extinction of many native fish.
Habitat fragmentation: Road construction through forests isolates species populations, e.g., tigers in India.
Habitat loss: Deforestation in the Amazon rainforest significantly reduces biodiversity.
Step 2 (b):
(i) IUCN Red data list: This list details global conservation status of species, aiding conservation prioritization.
(ii) Hot spots in conservation of biodiversity: Biodiversity hotspots, regions with high species richness and endemism under threat. Conservation in these areas maximizes biodiversity protection with limited resources.