Question

200 cm3 of an aqueous solution of a protein contains 1.26 g of the protein. The osmotic pressure of this solution at 300 K is found to be 2.57 × 10^-3 bar. Calculate the molar mass of the protein.

Given: R = 0.083 L bar mol^-1 K^-1

Hint:

Osmotic pressure is the preferred method for determining the molar mass of macromolecules like proteins because the pressure changes are measurable even at very low molar concentrations.

Answer 1