While separating the protein using polyacrylamide gel electrophoresis, the major ions playing an important role in stacking the protein are: i. Glycine of the running gel buffer ii. Chloride ion iii. The protein itself Which of the following is correct?
Show Hint
In SDS-PAGE, stacking occurs due to differences in ion mobility between the leading ion (chloride) and trailing ion (glycine).
ii moves faster than i, and i moves faster than iii
iii moves faster than i and ii
i moves faster than ii and iii
ii moves faster than iii and iii moves faster than i
Show Solution
The Correct Option isD
Solution and Explanation
1. Chloride ions (ii) demonstrate the fastest movement, attributed to their small size and high mobility. 2. Proteins (iii) migrate subsequently. Glycine (i) exhibits the slowest movement, a consequence of its zwitterionic form at the stacking pH.