Question

Which one of the following represents the correct annealing of primers to the DNA to be amplified in the PCR? 

• A. Both primers are complementary to the same strand.
• B. Primers anneal in a non-complementary manner.
• C. Both primers are complementary to the opposite strands at their ends.
• D. Primers anneal perfectly in opposite directions to the DNA template.

Hint:

Primer design is critical for PCR efficiency and specificity. Always ensure primers are complementary to their respective DNA template regions.

Answer 1

The Correct Option is D

For PCR to amplify a target DNA segment, primers must attach to complementary positions on opposing strands of the template DNA. This complementary binding is essential for replicating the DNA sequence located between the primers.