Question:medium

Which of the following steps are related to polymerase chain reaction (PCR)?
(A) Extension
(B) Annealing
(C) Propagation
(D) Denaturation

Show Hint

PCR involves four main steps: Denaturation, Annealing, Extension, and Propagation, each crucial for amplifying DNA segments.
Updated On: Apr 4, 2026
  • (A), (B) and (D) only
  • (A), (B) only
  • (A), (B), (C) and (D)
  • (B), (C) and (D) only
Show Solution

The Correct Option is C

Solution and Explanation

Step 1: PCR Fundamentals.
Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying specific DNA segments, involving a series of temperature-dependent stages.
Step 2: Core PCR Stages.
- Extension (A): DNA polymerase synthesizes a new DNA strand complementary to the template strand at an optimal temperature for enzyme function.
- Annealing (B): Primers (short DNA sequences) bind to their complementary sequences on the single-stranded DNA template at a lower temperature.
- Propagation (C): This term typically denotes replication; in PCR, it can refer to DNA segment amplification by polymerase during the extension phase.
- Denaturation (D): High temperature is applied to the DNA template to break hydrogen bonds between complementary strands, rendering them single-stranded.
Step 3: Summary.
Extension, Annealing, Propagation, and Denaturation are all essential components of the PCR process.
Final Answer: \[\boxed{\text{The correct answer is (3) (A), (B), (C) and (D).}}\]
Was this answer helpful?
0