- (A) Correct: DNA microarrays facilitate gene expression analysis by probing immobilized DNA sequences through hybridization.
- (B) Correct: Differential centrifugation separates cellular organelles based on their sedimentation rates.
- (C) Correct: Essential amino acids, not synthesized by humans, are acquired through diet, with microbes being capable of their synthesis.
- (D) Incorrect: Equilibrium constants are reaction-specific, but this statement does not align with the provided context.

In the above represented plasmid an alien piece of DNA is inserted at the EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?