Question:medium

Which of the following is the correct sequence of reagents used in the Gram staining process?
(A). Iodine
(B). Crystal violet
(C). Safranin
(D). Alcohol

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Understanding the correct sequence of staining in microbiological techniques is crucial for diagnosis and research.
Updated On: Jan 17, 2026
  • (A), (B), (C), (D)
  • (A), (C), (B), (D)
  • (B), (A), (D), (C)
  • (C), (B), (D), (A)
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The Correct Option is C

Solution and Explanation

Gram staining follows a precise sequence: Crystal violet, then Iodine, followed by Alcohol for decolorization, and finally Safranin as a counterstain. This method differentiates between Gram-positive and Gram-negative bacteria.
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