Question

Which of the following is not a step in Multiple Ovulation Embryo Transfer Technology $(MOET)$?

• A. Cow is administered hormone having LH like activity for super ovulation
• B. Cow yields about $6-8$ eggs at a time
• C. Cow is fertilized by artificial insemination
• D. Fertilized eggs are transferred to surrogate mothers at 8 - 32 cell stage

Answer 1

The Correct Option is A

Multiple Ovulation Embryo Transfer Technology (MOET) is a reproductive technique used in livestock breeding, particularly in cattle. This method involves several key steps:

• Hormonal Treatment for Superovulation: The donor cow is administered hormones to induce superovulation. Typically, Follicle Stimulating Hormone (FSH) is used to stimulate the production of multiple eggs. LH-like hormones are not used for this purpose, which makes option 1 incorrect.
• Artificial Insemination: The super-ovulated cow is then artificially inseminated to fertilize the multiple eggs produced due to superovulation.
• Egg Retrieval: The cow produces about 6-8 eggs as a result of superovulation.
• Embryo Transfer: The fertilized embryos are harvested and transferred to surrogate mothers. This is done at a specific stage, often at the 8-32 cell stage, to ensure the best chances of survival and proper development.

To determine which step is not part of the MOET process, let's analyze the options given:

1. Option 1: "Cow is administered hormone having LH like activity for super ovulation" - Incorrect for MOET as FSH, not LH-like hormones, is typically used for inducing superovulation.
2. Option 2: "Cow yields about 6-8 eggs at a time" - This corresponds with the expected result from superovulation; hence, it is a part of the MOET process.
3. Option 3: "Cow is fertilized by artificial insemination" - Artificial Insemination is indeed a step in the MOET process where the cow is fertilized.
4. Option 4: "Fertilized eggs are transferred to surrogate mothers at 8 - 32 cell stage" - This is a crucial step in the MOET process to ensure embryo survival and development.

Hence, the correct answer is that administering LH-like hormones is not a step in the MOET process. The hormones used are more aligned with FSH activity. Therefore, the correct choice is the first option.