Question

Which of the following are the types of column chromatography?
A. Paper chromatography
B. Affinity chromatography
C. Size exclusion chromatography
D. Ion-exchange chromatography
Choose the correct answer from the options given below:

• A. A, B and D only
• B. A, B and C only
• C. A, B, C and D
• D. B, C and D only

Hint:

To distinguish chromatography types, think about the physical setup. If the stationary phase is inside a tube or column, it's column chromatography. If it's on a flat sheet (like paper or a thin layer on glass), it's planar chromatography.

Answer 1

The Correct Option is D

Step 1: Understanding the Concept:
Chromatography separates mixtures and is categorized by the stationary phase's physical arrangement.


Column Chromatography: The stationary phase is contained within a column.

Planar Chromatography: The stationary phase resides on a flat surface.

Step 2: Detailed Explanation:
Classifying the techniques:


A. Paper chromatography: Uses a paper sheet as the stationary phase, thus it is planar chromatography.

B. Affinity chromatography: Employs a stationary phase packed in a column, making it column chromatography.

C. Size exclusion chromatography: Uses a column with porous beads, thus it is column chromatography.

D. Ion-exchange chromatography: Uses a column packed with charged resin, classifying it as column chromatography.

Step 3: Final Answer:
Consequently, B, C, and D represent column chromatography, while A does not. Option (D) is correct.