Step 1: The question targets which chlorine species actually does the killing of microbes during water chlorination.
Step 2: Chlorine in water yields two free forms, hypochlorous acid and the hypochlorite ion, but their germicidal power differs greatly.
Step 3: Hypochlorous acid is the dominant disinfectant; being neutral and small, it slips through the microbial membrane and is many times stronger than the negatively charged hypochlorite ion.
Step 4: Hence the active disinfecting form is hypochlorous acid, while the hypochlorite and chloride ions contribute little or no disinfection.
\[\boxed{\text{Hypochlorous acid}}\]