Question

What is the purpose of using HAT medium in hybridoma technology?

• A. To kill unfused B-lymphocytes
• B. To kill unfused myeloma cells
• C. To selectively allow growth of only hybridoma cells
• D. To increase the rate of cell fusion

Hint:

Remember the sequence of Hybridoma Technology: \[ \boxed{\text{B-Lymphocyte} + \text{Myeloma Cell} \longrightarrow \text{Hybridoma} \longrightarrow \text{HAT Selection} \longrightarrow \text{Monoclonal Antibody Production}} \] HAT medium does not increase cell fusion; it only selects successfully fused hybridoma cells by eliminating unfused myeloma cells and allowing hybridomas to survive.

Answer 1

The Correct Option is C