Question

What is the correct sequence of polymerase Chain Reactions? A. Annealing, B. Extension, C. Final extension, D. Heat-denaturation, E. Storage ($4^{\circ}\text{C}$) Choose the correct answer from the options given below:

• A. E, A, C, B, D \
• B. A, D, B, C, E \
• C. D, A, B, C, E \
• D. E, B, A, C, D

Hint:

Remember the core PCR triplet acronym: Denature $\rightarrow$ Anneal $\rightarrow$ Extend (D $\rightarrow$ A $\rightarrow$ B), followed by the final cleanup and cold storage steps.

Answer 1

The Correct Option is C