Question

What is the approximate incubation period in procedure of Widal Test for testing typhoid?

• A. 15 min at 37$^\circ$C
• B. 30 min at 37$^\circ$C
• C. 60 min at 35$^\circ$C
• D. 30 min at 35$^\circ$C

Hint:

Widal test → 30 min at 37$^\circ$C is the standard incubation for antigen-antibody reaction.

Answer 1

The Correct Option is B

Step 1: Widal Test Overview.
The Widal test is a serological diagnostic method for typhoid fever, a condition caused by Salmonella typhi. It identifies agglutinating antibodies targeting the O (somatic) and H (flagellar) antigens of Salmonella.
Step 2: Test Principle.
The test operates on an antigen-antibody reaction principle. Visible agglutination occurs when patient serum is combined with antigen suspensions.
Step 3: Incubation Requirements.
Optimal antigen-antibody reaction necessitates incubation of the mixture at 37$^\circ$C for approximately 30 minutes. This duration is recognized as the standard and broadly accepted protocol.
Step 4: Determination.
Consequently, the Widal test requires an incubation period of approximately 30 minutes at 37$^\circ$C.